FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907087
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25820.284647448370443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15450.041.0954442
GTGGTAT15600.041.0641
GCGCTAT400.007875008527.49804119
GATCGAC2500.026.39375120
GTATCAA59550.026.1315161
AGAGTAC35300.025.78997411
CAGAGTA37550.024.53762210
TATCAAC63750.024.316952
GGTATCA30300.024.226941
GTGTTAC1302.75395E-923.2637251
TCGACAC2650.022.83108122
TCAACGC68250.022.672114
ATCAACG68750.022.54723
AGTACGG40600.022.08458113
CAACGCA70300.021.971885
ACGGGAT8800.021.8782716
AACGCAG71150.021.7878516
GAGTACG41550.021.57963812
ATCGACA3350.021.34294321
CGGGGTA1951.8189894E-1221.1558417
Produced by FastQC (version 0.11.5)