FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624373
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA24630.1516277357478855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23310.14350152335701222No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC20680.12731066079034803No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT19240.11844570181848628No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG18360.11302822689123741No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT18360.11302822689123741No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT18270.11247416695549606No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT17690.10890355848071842No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC17440.10736450310365907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC40300.032.3934862
GTGGTAT41500.031.6028771
GTATCAA135350.026.2661611
AGAGTAC103700.024.61270511
CAGAGTA104700.024.29881910
CTTGAGT5050.023.9458582
GCTTGTA7800.023.2651391
TATCAAC153400.023.219282
GACAGGT4750.023.1538221
CTTGCGG2900.022.76092755
AACGCAG155050.022.7587816
GCTTGGC2850.022.1890811
ATCAACG160150.022.171993
TCAACGC158800.022.1694224
CAACGCA162700.021.7055745
AGACAGG5450.021.1889341
GCACCGC7300.020.72191610
TGAGTAC4400.020.611854
ACGCAGA175000.020.2283427
CGCAGAG175400.019.9984878
Produced by FastQC (version 0.11.5)