FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874006
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85370.4555481679354281No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61090.32598614945736565No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47550.2537345131232237No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.18543163682506886No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.1840442346502626No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.13014899632124977No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.12337207031354223No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.10896443234440018No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.10682996746008283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29200.058.3625832
GTGGTAT32000.053.5629161
GTATCAA115000.041.2369161
TATCAAC128050.037.271182
GGTATCA57750.035.645321
ATCAACG137250.034.989613
TCAACGC137400.034.8586164
CAACGCA141700.033.890785
AACGCAG148050.032.4945836
GTCGAGG21850.029.7586358
CGCAGAG164000.029.2824618
ACGCAGA168700.028.5170337
TCGAGGT21350.028.2682029
GCAGAGT170550.028.1351979
CAGAGTA81900.025.22154610-11
CGTCAGA3200.023.9053735
AGTCGAG33500.023.723017
GACAGGT12700.023.1075821
AGAGTAC78300.022.55425810-11
GAGTACG93000.022.05186312-13
Produced by FastQC (version 0.11.5)