FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874006
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35460.18922031199473216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC37600.044.451582
GTGGTAT39650.042.725081
CGGATAT507.0389756E-427.49639544
GTATCAA143800.026.9844461
AGAGTAC81750.025.50125711
CAGAGTA84550.024.88444510
TATCAAC158400.024.2965342
GGTATCA72550.024.0214983
CGACGGT1103.285768E-722.50245710
TCAACGC171900.022.307284
ATCAACG172600.022.2486613
CAACGCA174350.022.0568775
AGTACGG95150.021.62085713
AACGCAG178950.021.5211896
GAGTACG96000.021.51537112
TATCCGA650.003239955321.15446321
TACGGGG35200.020.47098515
GACAGGT7950.020.40941
TACGGGT9450.019.20844815
GTACGGG107100.019.0800514
Produced by FastQC (version 0.11.5)