FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277993
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58070.454384335438457No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48310.3780145900642648No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.23536905131718247No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT25870.20242677385556884No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT16210.1268395053807024No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA15320.1198754609767033No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG14400.11267667350290651No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG12830.1003917861834924No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.1003917861834924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14900.060.7587552
GTGGTAT16650.053.366231
GTATCAA62300.041.968321
TATCAAC70000.037.341572
GGTATCA31800.036.7654651
TCAACGC72450.036.017334
ATCAACG73950.035.3442233
CAACGCA74150.035.1915785
AACGCAG77650.033.7695476
CGCAGAG83950.031.3365738
ACGCAGA85650.030.7642147
GCAGAGT88600.029.6919339
TACCGAG755.906604E-428.3328515
CAGAGTA53500.026.65141510-11
GAGTACG61200.022.9857212-13
AGAGTAC52100.022.59558510-11
TCGACAC3400.021.87548322-23
GTACGGG64400.021.54660414-15
ACGGGAT12250.020.98943716-17
AGTACGG57300.020.21126212-13
Produced by FastQC (version 0.11.5)