FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277993
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55610.43513540371504383No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC15180.11877999331764727No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT14300.11189419660358078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8500.037.529842
GTGGTAT9950.032.9168781
ACCGTCG350.00410114631.42647224
GTATCAA51850.030.9997981
GGTATCA21500.027.3949221
TATCAAC59150.027.337592
TCAACGC61700.025.8846114
CAACGCA63450.025.6059285
ATCAACG62300.025.5952053
AACGCAG64850.025.0965146
CAGAGTA62300.024.71454610
AGAGTAC61450.024.65371511
CGCAGAG66800.024.3639058
ACGCAGA67950.023.9506287
GCAGAGT69850.023.341249
AGTACGG66350.022.4662213
GAGTACG68950.021.65470112
TCGACAC3400.021.02882822
TAACGGC804.438789E-420.62765736
GCCGACG2001.8189894E-1220.62765755
Produced by FastQC (version 0.11.5)