FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858032
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.5372759990303392No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.4049965502452123No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.25884815484737167No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT20880.2433475674566916No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.11363212560836893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12400.059.634852
GTGGTAT12600.058.3645821
GTATCAA49750.043.063691
TATCAAC55850.038.427382
GGTATCA25250.037.878691
AGCGTCA1451.8189894E-1235.1690373
AACGCAG62050.034.859676
CAACGCA62050.034.791185
TCAACGC65300.033.189774
ATCAACG65950.032.7982183
ACGCAGA69100.031.2994257
CGCAGAG69450.031.1435058
GCAGAGT70100.030.8547279
CGTCAGA1551.3460522E-1030.1583965
GACAGAT2700.026.7644881
GCAGCGT1755.529728E-1026.7195071
CAGAGTA46850.025.35094610-11
GACAGGT3250.024.8510111
CCTACAT1051.4682524E-424.290461
GTCTAGG1051.4682524E-424.290461
Produced by FastQC (version 0.11.5)