FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858032
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44140.5144330281388108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8700.039.188092
GTGGTAT9300.036.974871
GTATCAA45300.029.6347481
GGTATCA21800.028.0140631
TATCAAC51600.026.269322
CAGAGTA51300.024.59780510
CAACGCA55100.024.490885
AGAGTAC51000.024.31125511
AACGCAG55500.024.269096
ACCCTCG701.7995348E-423.57252748
TCAACGC57950.023.381264
ACGCAGA58000.023.2704037
CGCAGAG57900.023.2169958
ATCAACG59100.023.0219863
GCAGAGT59150.022.7741589
GTAGTTA650.003237116221.15606714
TACGGGG23200.021.10135315
AGTACGG57950.020.9297213
GAGTACG58950.020.89638712
CTTATTC2500.020.8961012
Produced by FastQC (version 0.11.5)