FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145103
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44720.3905325547134188No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.2786648886606707No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT28590.24967186357908416No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.1894152753071121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11600.054.982181
TGGTATC11400.054.820662
GTATCAA42450.041.668161
TATCAAC47950.037.0611952
TCAACGC51350.034.432684
ATCAACG51800.034.0515023
CAACGCA52100.033.8554275
GGTATCA21450.033.500251
AACGCAG54450.032.4723246
ACGCAGA61100.028.9317877
CGCAGAG61700.028.7193098
GCAGAGT65950.026.9329939
CAGAGTA39650.023.25624810-11
ACCGCGT1102.0179807E-423.1783058
AGAGTAC37300.022.55690410-11
GAGTACG46550.019.67209812-13
GATTAGA1100.005468934319.3245352
AGTACGG43350.019.21277412-13
GTATATG2754.989488E-918.5539861
AGCGTCA2302.9085822E-718.4794963
Produced by FastQC (version 0.11.5)