FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145103
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57900.5056313711517654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9000.033.9289051
TGGTATC9150.031.8499242
GTATCAA41350.029.3394831
ATTATCG400.00786430127.50631538
TATCAAC46550.026.0460932
ATCAACG48100.024.9661393
TCAACGC48200.024.85954
CAACGCA48950.024.761495
AGAGTAC44400.024.6447111
AACGCAG50000.024.1336696
GGTATCA19750.023.9580081
CAGAGTA46200.023.9215210
CGCAGAG51500.023.272668
ACGCAGA51850.023.2205837
GCAGAGT54350.022.0036339
CGGGATT3050.021.64242217
CGTAGAA1155.036072E-721.52480133
AGTACGG49750.021.39313313
TCGACAC2100.020.9535322
ACGGGAT8750.020.74490216
Produced by FastQC (version 0.11.5)