FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669688
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44210.2647800068036663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT37800.043.2085881
TGGTATC38200.043.0272982
GTATCAA145400.027.1181561
AGAGTAC86350.025.82926411
CAGAGTA87850.025.66844610
TATCAAC159100.024.6524492
GGTATCA72900.023.4123123
ATCAACG171950.022.7448433
TCAACGC173100.022.577864
CAACGCA175300.022.3101885
AGTACGG99250.022.25110413
GAGTACG99700.022.20584312
AACGCAG178450.022.0100966
CGGGTAA1851.8189894E-1120.81243317
TACGGGG38450.020.7428415
GTACGGG110350.019.88827314
GACGTCG700.00497471719.64438634
ACGCAGA201800.019.437267
CGCAGAG201100.019.4156428
ACGGGGT11900.019.41327916
Produced by FastQC (version 0.11.5)