FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273551
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21300.1672488969817463No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT13820.10851548151585605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21950.038.450992
GTGGTAT23350.036.750141
GTATCAA81150.025.6904681
TATCAAC89100.023.1412732
GGTATCA41450.022.2154833
AGAGTAC62050.021.94177811
CAGAGTA62700.021.80118810
TCAACGC95000.021.498134
ATCAACG95300.021.4592973
ACGTCGG650.003244046821.14922520
AACGCAG96950.021.123266
CAACGCA97300.021.1029535
GCTTGTA4150.019.88221
CCGAGCG856.6854426E-419.4113339
ACGCAGA109300.018.6625237
CGCAGAG109800.018.528928
CAGGTAT4750.018.5288161
GCAGAGT113750.018.058949
GTCGAGG9650.017.663848
AGTACGG77550.017.5562513
Produced by FastQC (version 0.11.5)