FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296819
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.227865261073442No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.1655589561843249No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.1144338569993191No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA13690.10556600419950664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10000.052.281691
TGGTATC11000.047.5159762
GGTATCA25500.040.5051841
GTATCAA45750.039.300111
TATCAAC51100.035.176022
TCAACGC54600.033.231184
ATCAACG54650.033.1230243
CAACGCA56100.032.418395
AACGCAG59650.030.5602826
CGCAGAG64650.028.0642268
ACGCAGA65450.027.915967
GCAGAGT70400.025.953129
CAGAGTA47550.024.66336410-11
GTGTAAG4300.023.7239671
GAGTACG56050.020.99977512-13
GTACGGG56500.020.64450314-15
AGAGTAC46150.020.53179610-11
GCTTGTA4300.019.7699721
AGTACGG51550.018.34052812-13
AGAGTCG19800.017.812445
Produced by FastQC (version 0.11.5)