FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040348
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28700.2758692283735827No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT15940.15321796168205254No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT12740.12245902332680988No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC11460.1101554479847128No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT10860.1043881470431048No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT10460.10054327974869948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8600.034.2281951
TGGTATC8650.033.0587272
GTATCAA45900.030.2076191
GGTATCA19750.029.1123891
TATCAAC52700.026.0350762
AGAGTAC51850.025.56037311
TCAACGC54300.025.260644
ATCAACG54500.025.1691513
CCGTATG550.001227870924.9951279
CAGAGTA53550.024.85162510
AACGCAG55550.024.6934076
CAACGCA56000.024.590795
CGCAGAG57550.023.8841658
ACGCAGA57550.023.8830177
GCAGAGT61300.022.4262989
AGTACGG58150.022.36881413
GAGTACG59400.021.85074412
TGCCGAC4200.021.61124455
AACGCTA650.003237787921.15582549
ACGGGGT9100.020.24817316
Produced by FastQC (version 0.11.5)