FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195468
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64540.5398722508674427No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.35400362033948213No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.240407940655877No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA19610.16403617662706155No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC13780.11526866465685405No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT13670.11434852292156712No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT13280.11108620222373161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19850.055.243752
GTGGTAT20550.053.5934641
GTATCAA65550.040.8687861
TATCAAC74400.036.333582
ATCAACG77950.034.6759723
TCAACGC78850.034.2801784
CAACGCA80000.033.78745
GGTATCA34500.033.2607733
AACGCAG81850.033.0237276
ACGCAGA91100.029.6681217
CGCAGAG92250.029.2982738
GCAGAGT95400.028.330889
CAGAGTA54700.025.48225410-11
AGAGTAC51950.023.27276810-11
GAGTACG60150.023.03208512-13
GACAGGT6200.021.9473531
AGTACGG59250.020.47712912-13
GTACGGG69950.019.62303214-15
CCGTCTT12800.018.26204784-85
AGCGTCA2102.4976016E-618.2142333
Produced by FastQC (version 0.11.5)