FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195468
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19300.16144304991852562No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT17550.14680443140259714No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13240.11075160522908184No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12920.10807482927188347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11500.033.004722
GTATCAA54750.029.761831
GTGGTAT13150.029.513141
TATCAAC63900.026.3417972
GGTATCA25300.026.2191471
AGAGTAC60800.025.23470511
CAGAGTA62350.024.6966110
TCAACGC66650.024.6689384
ATCAACG66850.024.6383273
CAACGCA68000.024.5824935
AACGCAG68050.024.242236
ACGCAGA70250.023.5623057
CGCAGAG70450.023.4173558
ACGGGTC4350.023.39121416
AGTACGG65650.022.62035213
GAGTACG66600.022.50038312
GCAGAGT73350.022.454979
TGCCGAC4450.022.25037255
CGGGGTC5500.021.50037817
CGCGGTA1801.0913936E-1121.38747443
Produced by FastQC (version 0.11.5)