FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701687
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10180.1450789312043689No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8350.11899892687195289No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT7030.10018712046824295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGC150.005779885755.00670629
TGGTATC17600.038.5736962
GTGGTAT18800.036.2680851
TCGGAGC350.00409182131.43912947
TTAAACG952.4883775E-928.95089722
CGGGATA1103.947207E-1027.50531417
CGTAGGA705.6973695E-627.50335328
ACAGACG805.185211E-727.4935471
TCCGGTC705.719523E-627.489638
TATAGCG400.00789380827.4837515
GTATCAA63100.027.4499781
CCGGTCT759.757719E-625.6624749
CGGGATT1850.025.27515417
TATCAAC69150.025.0412122
AGGACGG550.001224697725.00483116
AGAGTAC41550.024.95490611
CAGAGTA42200.024.3732710
GACAGGT2050.024.1406761
ACGTAGG801.605554E-524.06543527
CTCCGGT801.6153337E-524.046577
Produced by FastQC (version 0.11.5)