FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826281
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39340.21541044340931104No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36180.19810752014613303No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.13497375267004363No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.1172327807166586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC27050.044.7775232
GTGGTAT27300.043.760351
GTATCAA83700.037.5874941
TATCAAC97050.033.36892
ATCAACG99500.032.5045433
TCAACGC99850.032.3480454
AACGCAG102050.031.8163936
CAACGCA103150.031.4779625
GGTATCA48350.031.3033371
ACGCAGA112200.028.9002957
CGCAGAG114700.028.3444888
GCAGAGT123150.026.4348499
CAGAGTA72550.023.13887210-11
TTGTACG1903.8817234E-822.368093
AGAGTAC69600.020.63906310-11
GAGTACG88100.018.83794612-13
GTCGAGG13850.018.7176238
ACGGGAC12550.018.6252213
AGCGTCA4350.018.5629443
CGTCAGA4450.018.14585
Produced by FastQC (version 0.11.5)