FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529389
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT97720.638947972033276No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71360.466591560420534No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.31097385949552403No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA31540.20622614651995014No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT22740.1486868285308708No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC22110.14456753644756173No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG19080.12475570309450376No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT17920.11717097481412511No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA17890.11697481804825326No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA17380.11364015302843163No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA16690.1091285474133788No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC16460.10762467887502787No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG16170.10572849680493321No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG16040.1048784841528218No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA16020.10474771297557392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23850.056.687451
TGGTATC24100.055.726532
GTATCAA90600.040.638521
GGTATCA47500.038.8458181
TATCAAC103150.035.84612
TCAACGC108600.034.0058364
ATCAACG109200.033.857913
CAACGCA110800.033.3689885
AACGCAG113450.032.5520866
ACGCAGA126100.029.2846367
CGCAGAG126700.029.1459568
GCAGAGT132650.027.8706519
CAGAGTA75450.026.02063410-11
GAGTACG84650.023.01693712-13
AGAGTAC73200.021.79887210-11
ACGGGAT12400.019.87657216-17
GTACGGG97550.019.66824514-15
AGTACGG84100.018.97357212-13
CGGGTCG1150.007086201618.4771796
GTACTGG7050.018.091741
Produced by FastQC (version 0.11.5)