FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529389
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT28150.1840604319764298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23680.15483307386152248No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT20390.1333212152042417No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC19720.1289403807664368No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC17500.11442478009191907No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT16980.11102472948347347No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA16760.10958624653374649No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT16070.10507464091869367No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA15760.1030476876713511No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT15480.1012168911898804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14700.035.1983871
TGGTATC14900.034.1460842
GTATCAA79100.030.7232041
GGTATCA34500.028.4794391
TATCAAC90850.027.0625082
TCAACGC94950.025.649634
ATCAACG95300.025.5842743
AACGCAG96500.025.4956726
CAACGCA97050.025.4070075
TGCCGAC5700.025.09149655
AGAGTAC90400.024.88026811
ACGCAGA100100.024.5512857
CAGAGTA91500.024.5202610
CGCAGAG100200.024.30818
GCAGAGT104800.023.321369
AGTACGG99450.021.98312213
GAGTACG100700.021.81733512
ACGGGTC8200.020.4583916
ACGGGAT12300.019.89941616
CGGGTCA6950.019.78520817
Produced by FastQC (version 0.11.5)