FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810309
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36090.19935823110861184No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT22780.12583487128440504No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT19060.10528589318177173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC42350.036.016752
GTGGTAT43650.035.0147861
GTATCAA153200.026.2689821
AGAGTAC102750.024.7880411
CAGAGTA104500.024.7137310
TATCAAC167600.023.9246832
AACGCAG180600.022.1252216
ATCAACG180850.022.0940253
TCAACGC181700.021.9446894
GGTATCA80500.021.7181171
CAACGCA184850.021.6004665
AGTACGG119400.021.21623613
GAGTACG120100.021.09257712
GACAGGT6200.020.3951591
CGGGTCA5100.019.95467217
ACGCAGA205000.019.494477
CGCAGAG205450.019.3613368
CGGGATA4150.019.22042317
CGGGCTA1151.2098219E-519.13397617
TACGGGG41800.018.7534715
Produced by FastQC (version 0.11.5)