FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764420
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.3338774214756124No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56500.3202185420704821No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37260.21117421022205599No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.16271636005032813No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.12650049307987893No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.10774078734088256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25450.053.9371722
GTGGTAT26500.052.4697761
GTATCAA94800.038.1705631
GGTATCA51250.037.2528761
TATCAAC106750.034.118222
ATCAACG112450.032.5021743
TCAACGC113300.032.4083754
CAACGCA114050.032.1589055
AACGCAG118950.030.9413476
CGCAGAG131800.027.9569288
ACGCAGA132500.027.7771557
GCAGAGT137500.026.7995039
GTCGAGG15800.026.6295538
CAGAGTA70700.024.91919110-11
TCGAGGT18150.022.0121049
AGAGTAC68150.021.64175410-11
AGTCGAG23550.021.2949627
GAGTACG82700.020.99467712-13
GTATTAG6500.018.9710771
ACGGGAT16750.018.90311416-17
Produced by FastQC (version 0.11.5)