FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377638
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.5063049799013871No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.39402814335421754No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.242030727839889No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7600.2012509334336057No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC6600.17477054745549972No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA5770.15279182709367173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5850.055.953771
TGGTATC5850.055.2197842
GTATCAA19800.039.504551
TATCAAC21500.036.3761182
GGTATCA11000.035.5540961
GTAGATT500.002785116434.003763
TCAACGC23550.033.3901064
ATCAACG23700.033.1787763
AACGCAG24450.032.508716
CAACGCA24200.032.4932635
GTATATA802.299301E-531.8827481
ATGAGCG550.0044418630.912515
ACGCAGA27000.029.4345447
CGCAGAG27000.029.277148
TGACGTC600.006796209628.336475
GCAGAGT28600.027.7878559
CAGAGTA15700.025.71599810-11
GCCTTAT1001.0523424E-425.5061971
CGGTGCT1001.0542388E-425.4994439
GCTTGTA1201.2708542E-524.7976931
Produced by FastQC (version 0.11.5)