FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377638
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20680.5476143820272324No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT4800.12710585269490887No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA150.005785518354.9882162
CGTCCTT401.900048E-434.3721912
GTATCAA15000.030.4429381
TGGTATC3350.030.3666252
GTGGTAT3700.028.9956341
GTCCTAT705.6796744E-627.5086771
GTACGCT400.00786460627.50139222
GGTATGC400.0078747827.4941083
TAATCCG400.00787987127.4904675
TATCAAC17450.026.469972
GGTATCA6450.026.442451
ATCAACG18150.025.6005733
AGAGTAC17400.025.28528611
AACGCAG18450.025.180976
TCAACGC18250.025.1556594
TATCCTC550.00122750824.9913335
CAGAGTA17850.024.8018910
CAACGCA18850.024.7924635
ACGCAGA19550.023.7672847
CGCAGAG19700.023.5863158
Produced by FastQC (version 0.11.5)