FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656670
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT20900.3182724960787001No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT20610.31385627484124445No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC16620.25309516195349263No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC15850.24136933315059314No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA15290.23284145765757533No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA14930.22735925198349247No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT14890.22675011801970546No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT13820.2104557844884036No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG13590.20695326419662846No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA13490.20543042928716096No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA13350.20329846041390653No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA13180.20070964106781186No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT13140.20010050710402486No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC13020.19827310521266392No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA12040.18334932309988272No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGATA12010.1828924726270425No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTA11760.17908538535337384No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC11680.1778671174257999No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTG11480.17482144760686494No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCT11400.17360317967929098No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTCACTTCC11190.1704052263694093No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATT10730.16340018578585894No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTT10300.15685199567514885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10170.15487231029284115No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGAT9830.14969467160065178No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAAC9270.14116679610763397No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGA9240.14070994563479372No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAACGGG9130.13903482723437952No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTCACTTCCCCT8830.13446632250597712No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGG8730.13294348759650967No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAG8680.13218207014177594No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCT8650.1317252196689357No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGAAATCAAT8640.13157293617798896No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCATAAGGTAGGTA8160.12426332861254512No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGA7860.11969482388414274No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGA7640.1163445870833143No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCA7570.11527860264668706No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCA7000.10659844366272253No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATC6640.10111623798863965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACC257.867254E-443.984954
GTGGTAT29200.037.4757651
TGGTATC29300.037.342172
GACAGGT1600.032.6500551
CTGCTTA1900.028.94849218
TGTCCTC400.00786629827.50316225
GCTAGGA507.02709E-427.50106811
AGCTAGG507.02709E-427.50106810
GGAACCG400.00788093427.492696
TGCTTAA2200.026.25101919
GTATCAA99850.025.1404481
ACGCGAA550.001225716725.0009716
CAGAGTA66050.024.60731310
CTTAAAC2350.024.57729521
AGAGTAC65550.024.50133111
AGGAGTC901.4497982E-624.4360833
CGGGAGC801.6063837E-524.06343517
GTCTTAG801.6092295E-524.0579341
CGGGATT2500.023.10089717
TTAAACG2400.022.91930222
Produced by FastQC (version 0.11.5)