FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316547
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37250.28293710744849976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22650.042.85491
TGGTATC23150.042.7412722
GTATCAA92500.027.5272751
AGAGTAC57900.026.0312511
CAGAGTA59550.025.35520410
TATCAAC100950.025.2932912
GGTATCA44850.024.0335311
ATCAACG108950.023.4342693
TCAACGC109200.023.3051194
GACAGGT5600.023.07831
CAACGCA111400.022.8695435
AACGCAG113450.022.4571536
AGTACGG67800.021.94626413
ACGGGCG1155.0374183E-721.52472916
GAGTACG70200.021.31350312
CCTATAC1851.8189894E-1120.797893
ACGGGTC1751.8371793E-1020.43140816
TACGGGG28800.020.05486915
GTACGGG74000.019.99602114
ACGCAGA128450.019.8369357
Produced by FastQC (version 0.11.5)