FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443064
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.19853589307196354No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.1935465093717257No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18230.12632842341018832No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.10824190749682619No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20500.047.891222
GTGGTAT21050.046.048551
GTATCAA70350.038.0119171
TATCAAC77350.034.8358762
TCAACGC82650.032.6019944
GGTATCA40400.032.411761
ATCAACG83350.032.3791853
CAACGCA85000.031.7006445
AACGCAG85200.031.5253736
ACGCAGA95100.028.1988627
CGCAGAG96150.027.8909198
GCAGAGT104150.025.7485529
GTCGAGG11550.023.9174088
CAGAGTA65050.023.09616710-11
CGTCAGA3000.022.667135
AGTCGAG17600.020.7667487
AGAGTAC61700.020.4927210-11
GCTTGTA5750.019.2237781
GAGTACG78850.018.89292112-13
TCGAGGT13100.018.8165259
Produced by FastQC (version 0.11.5)