FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267921
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29290.2310080833111842No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.21365684455104064No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.13873104081405702No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15240.12019676304754004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76600.037.4085771
AACGCAG86800.032.8527376
TATCAAC88650.032.5986022
GTGGTAT24450.031.473121
TGGTATC24550.031.3325582
TCAACGC91950.031.2437934
ATCAACG92100.031.2390523
CAACGCA94400.030.3878925
ACGCAGA96600.029.4318667
CGCAGAG97100.029.1939288
GGTATCA43450.028.8650511
GCAGAGT105550.027.0178139
CGGGCTA1308.032621E-726.1526366
GTCGAGG10200.024.9998288
CAGAGTA64300.022.93620310-11
GACAGGT6800.021.2573971
AGACGCA1404.203371E-521.2490144
AGAGTAC62100.020.49784710-11
CGGGACT8750.020.3990554
AGTACGG83700.020.2081432
Produced by FastQC (version 0.11.5)