FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267921
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19600.15458376349946093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28450.030.148851
TGGTATC28600.029.9812622
GTATCAA92650.025.251231
GCTTGTA4400.024.992271
AGAGTAC66000.023.7103611
TTCCGCG1052.0947482E-723.57623146
CAGAGTA68000.023.21338510
TATCAAC102650.022.7037832
CCGTTAA2200.022.5028116
GGCCGAC1103.2860407E-722.50103255
AACGCAG103400.022.2458556
TCCGCGC1003.6081492E-622.00535247
CCGCGCA1003.6081492E-622.00535248
GCCGTTA2400.021.77441215
ATCAACG108050.021.3656373
CGCGGCC2200.021.25181411
TCAACGC108250.021.2499964
CAACGCA110200.020.8731525
ACGCAGA114350.020.1901117
CGCAGAG114700.020.1572348
Produced by FastQC (version 0.11.5)