FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674965
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.40772484499196254No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.3740934715133377No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.23186387442311823No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.21082574652018993No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.15882305008407843No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.14756320698110273No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC9210.13645151970842934No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA8110.12015437837517501No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC7430.11007978191461779No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA7420.10993162608431548No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC7300.10815375612068774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22450.036.914952
GTGGTAT23250.035.4751051
GTATCAA66050.035.2094651
ACAGGTA4050.033.5923081
GGCACCG4200.033.392489
GCTTGTA2850.032.81881
AACGCAG72550.032.746016
AGGCACC4650.031.0749178
CGGGCTA1101.8603532E-730.9087414
GTCGAGG10650.030.727358
TATCAAC76850.030.692562
ATCAACG78300.030.2870123
TCAACGC78650.030.2603054
AGAGGCA4650.030.1609486
CAACGCA79800.029.877485
ATTAGAG4850.029.7934763
TATTAGA5000.029.749662
ACGCAGA82550.028.7792027
CGCAGAG83550.028.3838798
AAGTACG1652.7830538E-1028.3435121
Produced by FastQC (version 0.11.5)