FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882292
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56420.2997409541133894No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.23880460629912892No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.1759556965656763No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.11799444507015916No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.1099723103535477No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA18900.10040950075758702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23700.053.0881461
TGGTATC24250.051.6991922
GTATCAA89000.041.694471
TATCAAC98300.037.6563382
ATCAACG103300.035.751393
TCAACGC103900.035.504034
CAACGCA105750.034.9231075
AACGCAG108750.033.920636
GGTATCA44400.033.315821
ACGCAGA121400.030.456897
CGCAGAG124250.029.622258
GTCGAGG15900.028.0665288
GCAGAGT132200.027.873779
GTACGGT957.454202E-526.841153
TCGAGGT16250.025.893459
AGTCGAG21700.024.4813447
CAGAGTA68950.023.91693510-11
AGAGTAC64550.022.3208910-11
GACAGGT10950.021.3502431
GAGTACG79650.020.49054512-13
Produced by FastQC (version 0.11.5)