FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882292
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50290.2671742747671456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29500.042.1284071
TGGTATC30100.040.635172
CGTACGA553.4891113E-530.00365435
ACGTACG653.2047465E-629.61820434
GTATCAA109600.027.9970471
AGAGTAC70700.026.68511
CAGAGTA74550.025.38068410
TATCAAC120900.025.2351112
GGTATCA52400.024.233033
ATCAACG128450.023.5586413
TCAACGC129450.023.3341874
CAACGCA130800.023.1984185
AACGCAG133250.022.8137386
AGTACGG83050.022.55181713
CGAAACG1003.622672E-621.99624630
GAGTACG86650.021.64603212
ACGCAGA150200.020.222537
CGCAGAG151750.020.0388818
GTACGGG93300.019.72052814
GCAGAGT158050.019.3138079
Produced by FastQC (version 0.11.5)