FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494797
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.33144905890698606No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.29001792654361286No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.18027595155184856No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.1606719523360085No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA7380.14915207650814374No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC6700.1354090667485858No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA6530.1319733143086963No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT6190.1251018094289173No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC6180.12489970634421795No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.119645026142034No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA5770.11661347987154329No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.115400861363347No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC5650.11418824285515068No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT5560.11236931509285626No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG5440.10994407807646368No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA5090.10287047011198533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11900.041.7847672
ACCGCTC450.001662315837.7769248
GTATCAA40050.036.9466741
GTGGTAT13400.036.4914971
GACAGGT2050.035.2609251
GACAGGG1750.034.0164221
GACAGTA500.002780867734.016421
GCTTCGT500.002780867734.016421
CTAACGA250.002789109233.9992326-27
AACGCAG45250.032.872196
TATCAAC47050.031.7049182
TCAACGC47800.031.0296344
ATCAACG48250.030.7402363
CAACGCA48750.030.5121335
GGCACCG2250.030.221549
ACGCAGA50050.029.719617
CGCAGAG50100.029.520298
ACAGGTA2600.029.4372861
GGTATCA22850.029.4014991
GCTTGCC1054.5006436E-628.3470171
Produced by FastQC (version 0.11.5)