FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827542
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61240.21658387390885797No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57820.20448856285777542No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC47170.1668233398478254No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA40870.14454250370109445No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35990.12728369728902347No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA35350.12502024726776825No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT34660.1225799652136025No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC33940.12003358393969038No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.11073221900859474No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA30630.10832730336101108No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC30110.10648825021874121No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT29420.10404796816457546No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA28920.10227964783546982No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC28740.1016430525169918No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA28720.10157231970382757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC45650.040.2164462
GTATCAA161000.038.7709771
GTGGTAT50250.035.7940521
AACGCAG178600.034.9781346
TCAACGC187500.033.5671544
CAACGCA189050.033.2245035
ATCAACG191050.033.0768933
GACAGGT9700.032.8772961
TATCAAC197550.031.7734382
ACGCAGA200200.031.2467237
CGCAGAG201750.030.9650828
GTCGAGG20000.029.5361048
GGTATCA88800.029.2094541
GCTTGTA15950.028.7918551
GCAGAGT220850.028.3260829
CAGAGTA135900.024.29839710-11
GGCACCG17550.022.5207029
TTGTACG3850.022.0764883
AGAGTAC134900.020.95008510-11
TACGGGG83550.020.6509652
Produced by FastQC (version 0.11.5)