FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610837
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41280.2562642899312594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC34000.044.135172
GTGGTAT35050.042.983171
GTATCAA132800.027.6575871
AGAGTAC77250.026.20439111
CAGAGTA79600.025.91289710
TATCAAC145850.025.1374052
TACGCGG550.001223971725.0097953
ACGGGCT3100.023.95503616
GGTATCA65250.023.9227943
GACAGGT5850.023.0274641
ATCAACG159250.022.9517773
TACGTTA2400.022.92421547
TCAACGC160050.022.8027114
CAACGCA163150.022.4031285
AGTACGG89300.022.39191213
GAGTACG90550.022.23467812
AACGCAG168550.021.7838826
CGGGATA5150.021.36227617
ACGGGAT18600.021.29336516
TACGGGG33700.021.2196515
Produced by FastQC (version 0.11.5)