FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424353
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.18738332421808357No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.172499373399712No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.11212108234405377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12800.049.802942
GTGGTAT14450.042.9532431
GTATCAA53850.042.630361
TATCAAC61150.037.6683432
GGTATCA26800.037.435891
TCAACGC65550.035.334384
ATCAACG65900.035.082233
CAACGCA66800.034.7368055
AACGCAG67550.034.288216
ACGCAGA74600.030.9338997
CGCAGAG78850.029.2686238
GCAGAGT84050.027.6095359
CAGAGTA49550.023.54522710-11
GTCGAGG8350.022.3959968
AGTCGAG12600.021.9238347
AGAGTAC46450.021.2736210-11
TCGAGGT8000.021.2507469
GAGTACG57600.020.10704212-13
GACAGGT9350.020.005621
GTCGAGT5300.019.2459588
Produced by FastQC (version 0.11.5)