FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928707
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.39404637407340776No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66490.34473872910711684No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46340.24026459177054885No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.1892978041765805No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30150.15632234445149004No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA27810.14418986398659828No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT25360.13148705324344237No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC23450.12158404568449226No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT21760.11282169868207041No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC39550.051.039982
GTGGTAT41600.048.238321
GTATCAA134250.038.097361
TATCAAC150650.034.5565452
GGTATCA72600.032.6769181
ATCAACG161050.032.3250163
TCAACGC161200.032.2949334
AACGCAG163750.031.7401186
CAACGCA166100.031.3422225
GTCGAGG22450.030.8572468
ACGCAGA185500.027.9727487
CGCAGAG186300.027.8540738
GCAGAGT193900.026.8534749
TCGAGGT25550.026.4513859
AGTCGAG34400.024.8313877
CAGAGTA101450.024.531110-11
GACAGGT10150.021.7811531
GAGTACG116300.021.16123612-13
AGAGTAC97600.021.07840510-11
AGCGTCA3250.020.9218443
Produced by FastQC (version 0.11.5)