FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558337
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCATCCTTTAAAGAAA9240.16549145050390712No Hit
GTGGATGCCTTGGCAGTTGGAGGCGATGAAGGACGTATTAACTTGCGATA8450.15134228969242589No Hit
GTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT7180.12859617041320923No Hit
CCTATGTATTCAGTTAACGATACCTGCAAGCAGGTGGGTTTCCCCATTCG5730.10262619170859177No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGC259.145297E-568.004523
TGGTATC4700.045.2157672
GTATCAA17550.044.573341
ACAGCCG603.1984946E-642.5142441
TATCAAC20100.040.1767962
CAACGCA20550.038.6765335
AACGCAG21000.038.0467386
TCAACGC21400.037.1403164
ATCAACG21800.036.653813
ACGCAGA22500.035.510297
GTGGTAT5900.035.308441
GTACGGA1008.029383E-834.0113951
TGGACTC500.002786856634.002265
TCGGGCT500.002786856634.002264
CTAGGGA500.002786856634.002264
CGCAGAG24250.032.9476938
CAGGTCT802.3040431E-531.8771132
ACCCTAG550.00443878430.9194491
GCAGAGT26350.030.4831669
ACGGGCC1452.1773303E-929.312293
Produced by FastQC (version 0.11.5)