FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558337
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8430.1509840830896036No Hit
GGCTCAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCATCCTTTAAAGAAAGCGTAATAGCT8210.1470438104585582No Hit
GTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCTTTAAAGGATGG7630.1366558189767112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5350.040.0767442
CGCGGGC358.6604094E-539.2869314
ATCACGC300.001928720436.6513826
CGCGCGG401.8999504E-434.37606412
GTGGTAT6500.033.8411371
GATCGTT350.00410261731.4210978
TGTCTTA453.7824322E-430.57019647
TCTCTAA400.00785134527.51317847
GCGCGGG507.026088E-427.50084913
CCGCGCG507.029769E-427.49838611
CAGGTCT805.178099E-727.4959241
TAGCTCC1004.316462E-927.4934620
TGCAGTC400.0078857527.4885376
CGGGATA507.044509E-427.4885354
GCAAGCC957.919698E-826.06511349
ACAGGCC858.791212E-725.8785171
GTATCAA19150.025.8447321
ACAGCCT759.751915E-625.6628631
TTACTAA651.0910678E-425.3740333
TCTCGAT1201.0822987E-925.21137239
Produced by FastQC (version 0.11.5)