FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149303
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.2831281220008997No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT28490.2478893729503882No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.2001212909041393No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.13051388537226477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11650.050.348762
GTGGTAT12250.048.585111
GTATCAA43450.040.4083821
GGTATCA23700.038.565711
TATCAAC47600.037.146852
TCAACGC50400.035.077034
ATCAACG50750.034.9188583
CAACGCA52300.034.046495
AACGCAG53250.033.5188986
ACGCAGA57900.030.9724127
CGCAGAG60100.029.7679398
GCAGAGT64150.027.8885929
CAGAGTA43200.024.73975410-11
GTCCTAA2101.72804E-1024.2925531
GTATTAG3850.022.084141
GAGTACG49500.021.59152812-13
AGCGTCA1805.7022953E-721.2485873
AGAGTAC41600.021.04335810-11
ACGGGGG8500.019.99953816-17
GTACGGG53400.019.93633814-15
Produced by FastQC (version 0.11.5)