FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037975
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67170.32959187428697606No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.3056465363902894No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41780.2050074215826985No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.16256332879451418No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.12885339614077698No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.11001116304174487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29300.056.0129471
TGGTATC29200.055.741952
GTATCAA106750.037.200411
GGTATCA57200.036.942741
TATCAAC117950.033.7964552
TCAACGC125400.031.754724
ATCAACG125750.031.7001343
CAACGCA127850.031.1802065
AACGCAG134150.029.8426326
GTCGAGG18600.027.8755728
CGCAGAG147550.026.9884098
ACGCAGA151550.026.3882487
GCAGAGT156850.025.4701149
TCGAGGT19450.025.3469649
AGTCGAG28000.023.0707917
CAGAGTA79700.022.90300210-11
GAGTACG88600.020.38625512-13
AGAGTAC76050.020.20210510-11
GTACGGG96750.018.36169814-15
AGTACGG86750.017.63661412-13
Produced by FastQC (version 0.11.5)