FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037975
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29480.14465339368736124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT40700.042.6287041
TGGTATC40450.042.3296622
GTATCAA130850.024.4174061
GGTATCA72850.023.9167963
AGAGTAC81550.023.84404411
CAGAGTA86350.022.7080410
TATCAAC143650.022.1744612
AGTACGG94000.020.54018813
GAGTACG94850.020.47210512
TCAACGC155350.020.4316484
ATCAACG155600.020.3816593
CAACGCA157900.020.1011945
AACGCAG161950.019.6178846
CGGGATA5600.019.1545417
GTACGGG102550.018.69357114
TACGGGG35300.018.62165815
ACGGGGG10200.018.60561616
CGATCTA909.827536E-418.33101720
ACGGGTA4400.018.12760716
ACGGGAT22750.017.89268316
Produced by FastQC (version 0.11.5)