FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024872
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23520.2294920731564527No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18000.1756316886401424No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13720.13387037600793075No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12840.1252839378966349No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC11220.10947708591902208No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA10930.10664746426870866No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT10870.10606202530657487No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT10810.10547658634444107No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA10560.10303725733555019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10150.036.0412671
TGGTATC9800.035.9023362
GTTATAC401.9096788E-434.3495983
GTATCAA47200.031.526061
TATCAAC54650.027.6637672
GGTATCA20300.026.9632031
ATCAACG57850.025.8883743
AGAGTAC54550.025.81099711
CAGAGTA54550.025.75681310
TCAACGC58350.025.7123784
AACGCAG58400.025.6470666
CAACGCA58950.025.639635
ACGCAGA59900.025.0978037
CGCAGAG60550.024.7376028
GCAGAGT63000.023.5655089
AGTACGG61300.022.61326413
GAGTACG62700.022.06124712
TGCCGAC4800.021.20294455
CATACCG700.004995579419.6293015
TACGGGG27300.019.5448515
Produced by FastQC (version 0.11.5)