FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774939
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28360.36596428880208637No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.28402235530796616No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16410.21175860293519877No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.16698088494707325No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.1441403775006807No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.11213785859274085No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.10220159264148533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12500.055.114851
TGGTATC12450.054.959132
GTATCAA44600.038.7128221
GGTATCA24350.035.9775771
TATCAAC49900.034.834222
TCAACGC52950.032.9882434
ATCAACG53150.032.7841453
CAACGCA53800.032.4670525
AACGCAG56350.031.0732486
TATACGT600.00680466528.3329684
ACGCAGA63700.027.621317
CGCAGAG63800.027.5780128
GCAGAGT66200.026.5782079
GTCGAGG7150.026.153518
CAGAGTA33000.025.05053510-11
TCGAGGT7700.024.28549
GTCTAAG1652.4475958E-723.1964861
ACAGGTA4800.023.0353981
GCGTCAG1502.7884853E-622.6663744
AGCGTCA1553.7063965E-621.9352023
Produced by FastQC (version 0.11.5)