FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774939
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20410.2633755689157469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12150.042.9768182
GTGGTAT12750.040.5415951
GTATCAA51400.028.6717911
GCCGTTA507.0240954E-427.50384315
ACGGGTC400.007868689527.50206616
TACGCGA507.0320535E-427.49851429
TATCAAC56700.025.7376212
CCGTTAA651.087959E-425.38652216
GACAGGT3450.024.7056221
AGAGTAC31750.024.16873211
ATCAACG60450.023.9575963
TCAACGC60800.023.8196814
CAACGCA61150.023.6384075
CAGAGTA32750.023.59871510
ACGCGAG701.8044881E-423.5625530
AACGCAG62550.023.0683736
GGTATCA24150.022.997861
TTAGCCC1155.0726703E-721.5066914
AGTACCG904.0150604E-521.3739345
AGTACGG35900.021.14501613
Produced by FastQC (version 0.11.5)