FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051450
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30700.2919777450187836No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.2855104855199962No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.18545817680346188No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.14351609681867897No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.112035760140758No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGT200.00322408563.7466133
GTGGTAT15150.053.8841481
TGGTATC15650.052.1378022
GTATCAA52750.040.3013881
TATCAAC58250.036.4057922
ATCAACG63250.033.460673
TCAACGC64050.033.042744
CAACGCA65100.032.5750735
GGTATCA28800.032.1837351
AACGCAG66650.031.7537526
ACGCAGA74050.028.580527
CGCAGAG74900.028.2561768
GCAGAGT79100.026.8108489
GTCGAGG8150.026.593688
TAGTACG1159.177233E-625.868194
ACTACGC900.001711497223.6098563
CAGAGTA39400.023.35329410-11
TCGAGGT8950.023.2680269
AGTCGAG12350.022.023127
AGAGTAC38650.021.2773710-11
Produced by FastQC (version 0.11.5)