FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051450
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16440.15635550905891865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19250.040.2606932
GTGGTAT19300.039.609411
AGAGTAC42650.026.1173811
GTATCAA68900.026.1017381
CAGAGTA43800.025.49444210
ACGAGTC550.001224461525.0070847
TATCAAC74250.024.2441922
TCAACGC77750.023.0092324
ATCAACG77950.022.9501973
CAACGCA79300.022.5941475
ACGGGCT2800.022.59245516
GGTATCA35600.022.5400243
AGTACGG49200.022.52856413
GAGTACG49650.022.37977612
AACGCAG81300.022.1703646
TACGGGC8200.021.13101215
CGGGATA2300.020.32893817
CGCAGAG90650.019.8949838
ACGCAGA90850.019.8436287
GTACGGG55500.019.72348814
Produced by FastQC (version 0.11.5)