FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152176
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27950.24258446626209884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22100.042.038812
GTGGTAT22900.040.587821
GTATCAA85050.026.80371
AGAGTAC55750.024.56719611
TATCAAC93600.024.3447112
CAGAGTA57050.024.15200410
CGGGCTA701.7995467E-423.57351317
GGTATCA42150.023.4865631
TCAACGC100750.022.5341974
ATCAACG101400.022.4168553
CAACGCA103100.022.0205675
AACGCAG104650.021.748836
CGGGATA3200.021.48627517
AGTACGG64350.020.9847613
GAGTACG65600.020.7945212
GTCCAAT1501.4319085E-820.1658231
ACGGGCT3050.019.8378216
GACAGGT4800.019.4783521
ACGCAGA118750.019.262387
CGCAGAG119350.019.074238
Produced by FastQC (version 0.11.5)