FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984594
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27140.27564661169984783No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG11890.12076043526570343No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT10620.10786171762167959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22750.044.6055071
TGGTATC23200.043.7158782
TAGCGTG350.004111101431.4101965
ACGTCCG805.183938E-727.49649443
GTATCAA90200.027.2262921
AGAGTAC54800.025.6953811
TATCAAC96850.025.1156522
CAGAGTA57100.024.80485710
ACGGGTC1451.4551915E-1124.6557916
GGTATCA44200.024.126163
ATCAACG104150.023.1957443
TCAACGC104400.023.0612224
CAACGCA106750.022.6565365
GACAGGT3550.022.465351
TACGGGT4550.022.36319415
AACGCAG108800.022.3306876
ACGGGGC3950.022.2790416
GACGTCC1003.6152214E-621.99943242
AGTACGG63950.021.80384313
CGGGATC6850.021.6793717
Produced by FastQC (version 0.11.5)