FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662412
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31210.18773926078493178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19700.037.5224382
GTGGTAT20050.036.750281
GTATCAA75350.025.724451
GGTATCA39300.024.5558411
TATCAAC82100.023.295452
AGAGTAC52900.022.77275511
CAGAGTA54600.022.41632810
ATCAACG88300.021.6273353
TCAACGC88300.021.533984
CAACGCA89250.021.3971275
AACGCAG91550.020.9502416
AGTACGG60250.019.81326113
ACGCAGA101050.018.9568777
GAGTACG63550.018.91368112
CGCAGAG102550.018.5517358
TACGGGG25050.018.33715815
GCAGAGT108000.017.6701959
GTACGGG67550.017.42776514
TACCCCG1454.6784608E-617.0550655
ACGGGTA2507.2759576E-1116.50344316
Produced by FastQC (version 0.11.5)